If you are a new user, please contact the Core directly to request services and specific pricing information. If you are a current user, please login to VPN to access pricing. Location of Service: Karp Family Research Building Room 08016 Pricing
Flowjo price cytometry software#
We are equipped with two data stations with several popular analysis software available to researchers. Location of Service: Karp Family Research Building, Room 08016 Data analysis: Flowjo, Modfit, FACS Diva, Kaluza Researchers are offered training for performing analysis independently. Our facility has 5 analytical flow cytometers that are available 24*7 for investigators to meet their research needs. Location of Service: Karp Family Research Building, Room 8113, 6113 and Center for Life Sciences, Room 15037 Cell analysis Training will be provided for researchers willing to sort independently upon requirement. Our experienced staff members are dependable, flexible, and prepared to assist with your flow cytometry experiments. Our facility is equipped with 4 sorters 3 of which are maintained for BL2 level sorting within HEPA filtered safety cabinets. Location of Service: Karp Family Research Building, Room 8113 Cell sorting Our lab offers training and access to shared user instrumentation for analytical flow cytometry, service for fluorescence activated cell sorting (FACS) Location of Service: Karp Family Research Building, Room 8113 Instrument training Our lab offers consultation on experimental panel design and helps the users choosing the right antibodies and fluorochromes required for their experiment. Location of Service: Karp Family Research Building, Room 8113 Panel design eBiosciences New Fluorochrome Options = Repackaged.The Flow Cytometry lab provides consultation on experimental design and data interpretation.If you have any questions/problems, send me an email, or submit your bugs directly to folks at FlowJo. The gates must be 'nearby' meaning that you'll have to spend some time (albeit, much less) to get the gates close, but then, voila! it just works. The couple of tries I've made thus far have worked really well. Now, if you select a bunch of gates (shift-click them, or cmd+a to select all of them) you can hit cmd+opt+m to 'Mold' the gates together. This gets back to the point of not being able to get all my polygons to match up exactly with no whitespace and no overlap. The second new gating function is what they call molded gates. A small price to pay, I think, but hey guys, if you're listening, that'd be nice. You need to do a select all gates function (cmd+a) and then grab the center. Wonderful! My only complaint in actually using this is that after you convert your standard quad gates into a spider gate, you can no longer simply grab the center point and move all 4 quads at the same time. We’re here to help you accelerate routine phenotyping, take your immunology research to the next level, and get you from data to resultsone cell at a time.
Just option-click the center point of the quads and move to bend the quad lines. FlowJo is your biggest fan and strives to be an outstanding source of support.
Flowjo price cytometry mac#
But now, FlowJo has introduced in the Mac version 8.8 (go figure, the Windows version falls behind again) 'Spider Gates'. And, when you attempted to do this, it was nearly impossible to get all the gates to line up without any whitespace inbetween (more about that in a minute). It was nice being able to easily match that slope with a set of quadrants in DiVa, but that wouldn't translate into FlowJo, so you were forced to create individual polygons for each of the populations. The slope of the line will depend on the amount of spillover and the spread that ensues. Therefore, it only makes sense that the line you draw to differentiate between single positives and double positives should have a positive slope to it.
The positive fraction is always a bit higher than the negative fraction. One of the features I like best on the DiVa 6 software is the ability to bend quadrant lines to account for the spread that is revealed after you compensate. FINALLY!!! This has been on my list of FlowJo improvements for a long time.
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